Cell3™ Target: Actionable Mutation Panel (EGFR)
Detect actionable mutations in the EGFR pathway in primary tumor or liquid biopsy.
Targeted enrichment of the key oncogenes in the EGFR pathway provides a quick and sensitive method
for monitoring even the lowest frequency variants
Sensitive. Accurate. Affordable.
Clinically relevant content
A highly focused panel covering the actionable mutations in key oncogenes in the EGFR pathway.
Primary tumor or liquid biopsy
Developed for and validated on ctDNA as well as gDNA and FFPE giving you the option to profile primary tumors or liquid biopsies and matched samples.
Confidently call low frequency variants
Cell3™ Target error suppression technology including unique molecular identifiers (UMIs), enables you to accurately call ultra-low frequency mutations down to 0.1% VAF.
Fast and affordable
Using a focused panel simplifies analysis and reduces costs making this a fast and affordable method of profiling variants in the EGFR pathway.
Focused coverage of EGFR pathway
We know that a fast and affordable panel for profiling pathways like the EGFR pathway is important to you so the Cell3™ Target: Actionable Mutation panel (EGFR) only enriches for actionable mutations in the most relevant oncogenes. It offers a simple, fast and cost-effective tool to enable early cancer detection, treatment selection and patient monitoring in cancers like non-small lung cancer.
Table 1. Gene content for Cell3 Target: Actionable Mutation Panel (EGFR)
Sensitive enough for liquid biopsy
Liquid biopsy offers a non-invasive method of detecting cancer early or monitoring cancer post chemotherapy or surgery, but it requires extremely sensitive analysis due to the small amounts of cell free (cfDNA) and even smaller amounts of circulating tumour (ctDNA) present. The error suppression technology of Nonacus’ Cell3™ Target libraries includes unique molecular indexes (UMIs) and unique dual indexes (UDIs) for removal of both PCR and sequencing errors and index hopping events. This ensures that the Actionable Mutation panel (EGFR) will enable you to confidently call mutations down to 0.1% VAF from as little as 10ng ctDNA input.
Maximise sequencing efficiency
By increasing the yield per sample, Nonacus Cell3™ Target library preparation solutions allow you to run more samples per flow cell increasing your efficiency and reducing your cost per sample.
Table 2. No. of samples per flow cell to achieve 100x mean depth of coverage based on 2 x 100bp library and maximum quoted sequencer output for Nonacus Cell3™ Target: Actionable Mutation panel (EGFR)
Product Resources
Detailed product information available to download.
Cell3™ Target Protocol v1.2.2
Cell3™ Target Experienced User Guide v1.2.2
Cell3™ Target De-Multiplexing Guide v1.0
NextSeq, MiniSeq, HiSeq3000 and HiSeq 4000 Example Sample Sheet 384 Indexes
MiSeq, NovaSeq, HiSeq2000 and HiSeq2500 Example Sample Sheet 384 Indexes
Nonacus 1-384 UMI Adapters Plate Format
For Research Use Only. Not for use in diagnostic procedures.
Product Support
If you have any questions about any of our products, including access to the BED files and example data sets, please fill in the support request form here and we will get back to you as soon as possible.
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