VirPath: Respiratory Multiplex qRT-PCR Kit

Differentiate COVID-19, flu and RSV in one assay

Highly sensitive and simple to use real-time RT-PCR assay designed for simultaneous detection of SARS-CoV-2, influenza type A/B and Respiratory syncytial virus (RSV) subtypes A and B.

True multiplex assay

Streamline testing by detecting Flu A/B, RSV A/B and SARS-CoV-2 in a single, real time RT-PCR reaction

Workflow flexibility

Choose the RNA extraction method and PCR thermocycler that fit with your workflow


Highly sensitive

Detect as little as 5 copies of viral RNA per reaction and pick up infections with low viral load

Robust performance

Have confidence in your results with an assay that targets conserved regions of SARS-CoV-2 and delivers minimal inter and intra assay variation.

A multi-target detection assay for respiratory viruses

As the symptoms of respiratory infections like COVID-19, flu and RSV can be very similar, it is hard to make an accurate diagnosis based on symptoms alone. The treatment, and risk from co-infection, of SARS-CoV-2, influenza, and RSV are different, so it is important to distinguish between them to gain a clear picture of infection, avoid inappropriate prescriptions and reduce the rate of transmission within the community.

As public health guidance changes and social mixing increases, co-circulation, transmission and cases of respiratory viruses are also likely to increase.  Increases in transmission are also likely to result in more co-infections which have been shown to be associated with worse outcomes for severe COVID-19.

Respiratory multiplex qRT-PCR kit (CE-IVD)

The VirPath Respiratory Multiplex qRT-PCR kit (CE-IVD*) is a highly sensitive and simple to use real-time, reverse transcription PCR assay that enables the simultaneous detection of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), influenza virus type A or B (Flu A/B) and Respiratory Syncytial virus subtypes A and B (RSV A/B).

It contains all the reagents and controls needed including an endogenous control, RNase P (RPP30), which confirms the presence of human biological material in a sample so you can be sure that a negative result is due to absence of virus and not missing sample.



Flu testing, woman sneezing with tissue over her nose
Nonacus Single test for SARS-CoV-2, influenza A/B and RSV A/B

Single test for SARS-CoV-2, influenza A/B and RSV A/B

Our VirPath Respiratory Multiplex qRT-PCR kit is a true multiplex assay that allows laboratories to accurately detect SARS-CoV-2, Flu A/B and RSV A/B in a single reaction, in a single well.

It allows your laboratory to streamline workflows, to test and differentiate between multiple viruses, saving time, conserving reagents and resources and reducing costs.



Workflow flexibility - run your lab your way

The VirPath Respiratory Multiplex qRT-PCR kit is suitable for use with any purified, high quality RNA independent of swab type or extraction method used. Developed with fluorescent reporter dyes: FAM, HEX, ROX and Cy5, the assay was validated on the BioRad CFX96 but, with appropriate validation, should be compatible with any equivalent real-time PCR systems.

Nonacus VirPath Respiratory Multiplex qRT-PCR kit
Covid 19 cell

Designed to protect against emerging variants

The VirPath Respiratory Multiplex qRT-PCR kit uses primers and probes that are designed to detect multiple variants within both the A and B subtypes for influenza and RSV. It also targets two highly conserved regions of SARS-CoV-2, (ORF-1 and N) and not the spike protein where many of the variants of concern have arisen.

We continually monitor the public genomic databases for all new variants, so as our customer, you can always be confident of our product performance.


*The VirPath Respiratory Multiplex Kit is a ‘Self-Declared’ Class 1 product under MDR2002 MHRA registration.


Product Specifications

Product Specifications.
  • SARS-CoV-2: Nucleocapsid gene (N) and Polymerase gene (ORF-1)
  • FluA: Matrix protein gene (M)
  • FluB: Non-structural 2 gene (NS2)
  • RSV A and RSV B: Nucleocapsid gene (N)
Internal control RNAse P (RPP30) gene, confirms presence of human material and acts an extraction control
Other controls Full process control to confirm validity of end-end processing as well as plasmid controls for confirming assay and RT-PCR functionality
Fluorescent dyes
  • SARS-CoV-2 - FAM
  • Influenza A/B - HEX
  • RSV A/B - Texas Red (ROX)
  • RNase P - Cy5
Sample type Nasopharyngeal or oropharyngeal swabs
RNA extraction Compatible with RNA or total nucleic acid extraction products or systems that deliver clean nucleic acid isolation (ie free of PCR inhibitors), but should be validated prior to use.
PCR Thermocycler Performance has been verified using the BioRad CFX96, controlled by the CFX software. The assay should be compatible with other equivalent real-time PCR cyclers, but validation should be performed prior to use.
Limit of Detection (LOD)
  • SARS-CoV-2: 5 copies per reaction
  • Influenza A: 20 copies per reaction/Influenza B: 10 copies per reaction
  • RSV A/B: 10 copies per reaction
  • SARS-CoV-2: 100% (n=173)
  • Influenza A/B: 100% (n= 137)
  • RSV A/B: 100% (n=137)
  • SARS-CoV-2: 99.08% (n=173)
  • Influenza A/B: 98.95% (n=137)
  • RSV A/B: 100% (n=137)
Cross Reactivity No cross-reactivity was detected between the assays included in the VirPath respiratory multiplex kit and common human viruses:
  • Human Parainfluenza 1
  • Human Parainfluenza 2
  • Human Parainfluenza 3
  • Human Parainfluenza 4
  • Human Adenovirus
  • Human Bocavirus
  • Human Coronavirus HKU-1
  • Human Coronavirus CORV OC43
  • Human Coronavirus CORV 229E
  • Human Coronavirus CORV NL63
  • Human alphaherpesvirus 3 (HHV-3)
  • Human Metapneumovirus A
  • Human Metapneumovirus B
  • Human Rhinovirus
  • Human Parechovirus
  • Human Enterovirus

Product Support

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Ordering Information

VirPath - Respiratory Multiplex qRT-PCR kit, 96 reactions C3COV195

Learn more about our products for COVID-19

Nonacus VirPath Respiratory Multiplex qRT-PCR kit

SARS-CoV-2 testing products

Complete testing workflow for SARS-CoV-2 (COVID-19) from viral RNA extraction to highly sensitive and accurate multiplex qRT-PCR