An efficient cell-free DNA (cfDNA) extraction kit
Cell3™ Xtract (RUO)
The Cell3 Xtract enables fast and flexible cfDNA extraction from a range of biological samples ensuring high quality cfDNA and circulating tumor DNA (ctDNA) for research applications.
Fast and flexible
Cell3 Xtract has a simple and flexible protocol that requires no specialist equipment and, with just 90 minutes of processing time, supports efficient your cfDNA extraction workflows.
Maximise sample input
Whether you are working with 1 ml or 10 ml you can extract all cfDNA in one extraction. With a small elution volume of just 35 µl you can avoid the need for a DNA concentration step.
Supports multiple biological sample types
Isolate cfDNA from a wide range of sample types: plasma, saliva and amniotic fluid.
Accurate quantification
Efficient cfDNA extraction without carrier RNA ensures accurate cfDNA quantification for sensitive downstream applications such as next generation sequencing (NGS).
Optimising cfDNA extraction from plasma
The utility of cfDNA in research has increased dramatically since its discovery. These short fragment sizes of ~160 bp are typically found in low quantities of 1-100 ng/ml in plasma. It is therefore critical that sufficient good quality cfDNA is extracted from biological samples for downstream applications like NGS and qPCR.
To address this need, Cell3 Xtract has been developed as a fast, accurate and flexible cell-free DNA extraction kit designed to optimise extraction and maximise cfDNA yield.
Large volume cfDNA extraction
Cell3 Xtract has a simple and flexible workflow which allows 1-10 ml of a biological sample to be processed within 90 minutes.
The flexible input volume allows for increased recovery and concentration of cfDNA from an entire sample, avoiding the necessity for multiple 1 ml extractions. No specialist equipment, such as magnets or vacuum manifolds, is required.
Obtain higher cfDNA concentration
With a small elution volume of just 35 µl, regardless of your starting volume, you can avoid the need for a DNA vacuum concentration step. This means you can add more cfDNA to your downstream applications including ddPCR and NGS for enhanced analytical sensitivity.
Accurate quantification of cfDNA
Accurate quantification of cell-free DNA extraction is important for downstream applications, particularly NGS where input amounts for assays can be critical.
The Cell3 Xtract cfDNA extraction kit does not require carrier RNA to optimize cfDNA isolation enabling accurate quantification of cfDNA and efficient library preparation.
Superior technical performance
We compared Cell3 Xtract to spin column and bead-based cfDNA extraction kits from four other companies.
Using cfDNA extracted from 1 ml plasma from a research donor, qPCR, DNA concentration and fragment size analysis showed that, in the majority of cases, Cell3 Xtract generated comparable or improved analytical performance relative to other manufacturers.
Comparison to spin-column based kits
Figure 1: Column-based cfDNA extraction kits: qPCR data revealed that the Cell3 Xtract kit performed on par with the Company Q kit and outperformed the Company A kit. However, the use of carrier RNA in the Company Q kit resulted in a 50-fold higher DNA concentration measurement and the appearance of a high molecular weight peak in the fragment analysis electropherogram.
Comparison to bead-based kits
Figure 2: Bead-based cfDNA extraction kits: qPCR and DNA concentration data showed that the Cell3 Xtract kit performed better than the Company M kit and on par with the Company O kit.
For Research Use Only. Not for use in diagnostic procedures.
