Cell3™ Target: Nexome - a clinically enhanced exome panel
Detect SNVs, indels and CNVs in a single, clinical-grade assay
Capture more variants and increase your diagnostic yield without increasing your sequencing costs.
Clinically relevant content
Maximise your diagnostic yield with coverage of important clinical targets from the major genetic databases including CNVs and non-coding regions associated with prenatal and postnatal disease.
Validate and run one workflow for all variants including SNVs, Indels and CNVs in a single, clinical-grade assay giving you the confidence to replace your array and MLPA-based CNV analysis.
Robust calling of variants
Confidently call SNVs, indels and CNVs with high recall and precision from as little as 1 ng of DNA, unlocking prenatal or limited samples, without compromising on quality or robustness.
Maximise content, minimise sequencing costs
Superior hybridization capture performance delivers significantly more content without increasing the amount of sequencing per sample or your sequencing costs.
Exome sequencing for clinical genetics
Current clinical genetic workflows often involve undertaking multiple tests with different technologies such as chromosomal microarrays (CMA), multiplex ligation probe amplification (MLPA), fluorescent in situ hybridization (FISH) all often followed by NGS (exome sequencing). This increases associated test cost, time to result and has negative implications for the required amount of sample input and the cost to process a given sample.
Cell3™ Target: Nexome has been developed combining our experience of exome panel design and input from experts in the field. As well as maximising coverage of clinically relevant targets for SNVs and indels, Nexome is designed to cover exon level deletions and duplications providing an exome alternative to CMA and MLPA based CNV analysis. It is a clinically enhanced human exome capture kit that allows you to carry out robust whole-exome sequencing and targeted copy number analysis in one single test.
Comprehensive coverage of clinically relevant regions
Larger than most exome products, Cell3™ Target: Nexome targets not only the protein coding regions of the human genome but clinically relevant non-coding regions. It is designed to offer excellent coverage of CCDS, ClinVar, GENCODE, RefSeq and ACMG73 databases (Figure 1) with enhanced probes to enable CNV detection at loci with known gene and exon level rearrangements.
Figure 1. Coverage of different databases by Nexome compared to other commercially available kits.
Figure 2. Percentage coverage of gene transcripts and variants by Nexome compared to other commercially available kits.
Increased diagnostic yield for exomes
Optimising diagnostic yield from a genomics test is important so our Nexome panel has been designed to cover clinically relevant coding and noncoding regions with no loss of coverage across the wider exome. Boosted regions of the Nexome panel include:
- Additional RefSeq transcripts across the Online Mendelian Inheritance in Man (OMIM) morbid set of genes.1
- Genes associated with pre and postnatal phenotypes (fetal anomalies).
- Transcripts and extra exons associated with Early Infant Epileptic Encephalopathy (EIEE) genes for enhanced epilepsy diagnosis.
- Promoter, 5’ and 3’ UTR sequences for current OMIM morbid genes.
- Non-coding, disease-causing variants as reported by Smedley et al. 2,3
- Pharmacogenomic (PGx) markers for drug response prediction.
Precision and recall of SNVs and indels
Nexome detects significantly more truth variants present in the HG001 human genome reference standard than other exome captures, and maintains superior precision and comparable recall for both SNVs and indels (Figures 3 and 4).
Figure 3a: Precision and recall of SNVs detected by Nexome and other commercially available exome panels.
Figure 3b: Total number of truth variants (SNVs) detected by Nexome and other commercially available exome panels.
Figure 4a: Precision and recall of Indels detected by Nexome and other commercially available exome panels.
Figure 4b: Total number of truth variants (Indels) detected by Nexome and other commercially available exome panels.
Precision CNV calling from exome sequencing
Copy number variants account for ~10% of curated disease associated variants and are identified in ~10–20% of individuals with neurodevelopmental disorders.
Enhanced coverage across loci with known gene and exon level rearrangements means that Cell3™ Target: Nexome is capable of detecting CNVs with sizes spanning from just a few exons up to multiple contiguous genes (~100 bp–40 Mb); detection of clinically relevant events is achieved with superior precision and recall and provides an exome alternative to CMA and MLPA based CNV analysis. (Table 1a and 1b)
|CNV exons||CNV type||Bayes
Table 1a. Detection of MLPA-confirmed CNVs
|CNV region||CNV size||CNV genes||CNV type||Bayes factor|
Table 1b. Detection of CMA-confirmed multi-gene CNVs.
Deliver more content without increasing sequencing costs
Our Nexome panel targets just over 51Mb of the human genome. This large capture size ensures that you can call up to 30% more variants than other commercially available exome panels and detect a wide range of CNVs, but our probe design and superior performance (Table 2) mean that despite being larger, Nexome requires a similar amount or less sequencing to achieve a mean coverage of 100x.
That means you can detect more clinically relevant variants without increasing your sequencing costs.
|Panel Size (Mb)||Percentage target covered at 1x||Gb Required for mean 100x coverage||Percent Bases on or near bait|
Table 2. Gb of sequence required to achieve mean coverage of 100x for Nexome and other commercially available exome products. Data was down sampled to 100x per sample for each exome panel.
Simple, automatable protocols
The Cell3™ Target Nexome kit contains all reagents for both library preparation and hybridization and capture. The Cell3™ Target workflow is simple and easy, requires as little as 1 ng of DNA and takes less than 10 hours, with less than 2 hours hands-on time. It is designed with multiple stop points to provide flexibility within laboratory processing.
Library preparation can be run manually or automated (up to 96 samples in a single batch). Indexes are available for up to 384 samples to facilitate high through put laboratories, allow for flexible batch sizes and provide scalability across all Illumina sequencers.
- Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), 2018.
- Smedley D et al. (2016). A Whole-Genome Analysis Framework for Effective Identification of Pathogenic Regulatory Variants in Mendelian Disease. Am J Hum Genet. 99(3): 595-606 (2016).
- Landrum, MJ et al. (2018) ClinVar: improving access to variant interpretations and supporting evidence, Nucleic Acids Research, Volume 46, Issue D1, D1062–D1067 (2018).
|Enrichment method||Hybridisation and capture|
|Capture Panel Size||51.9 Mb|
|Targets||Clinically relevant genes|
|Variant types||SNVs, indels and CNVs|
|Sample type||gDNA from blood, saliva, amniotic fluid, tissue or FFPE, cfDNA|
|Input DNA requirements||1-1000 ng|
|Expected percentage duplication||~5-6%|
|Expected percentage on target (150bp padding)||~95%|
|Gb required for mean 100x coverage||6.63|
Cell3 Target panels are available with one of two versions of our library preparation kits:
-Fragmentation: for use with gDNA (FF or FFPE)
-Non-Fragmentation: for use with cell free DNA
|Cell3™ Target: Nexome, Frag 16 samples||NGS_C3T_NEX_FR_16|
|Cell3™ Target: Nexome, Frag 96 samples||NGS_C3T_NEX_FR_96_A/B/C/D*|
|Cell3™ Target: Nexome, Non Frag 16 samples||NGS_C3T_NEX_NF_16|
|Cell3™ Target: Nexome, Non Frag 96 samples||NGS_C3T_NEX_NF_96_A/B/C/D*|
* To provide flexibility in multiplexing samples, our 96-sample kits offer a choice in adapter plate:
A = Adapter plate with indexes 1-96
B = Adapter plate with indexes 97-192
C = Adapter plate with indexes 193-288
D = Adapter plate with indexes 289-384
Detailed product information available to download.
For access to the BED files for this panel design, please visit My Nonacus
FASTQ files for example data sets are available on request, please email firstname.lastname@example.org or complete our support request form.
If you have any other questions about this panel or any of our products, including the Fetal RhD Screening Kit, ExomeCG, NGS Panels, or Whole Exome Enrichment, please fill in the support request form here and we will get back to you as soon as possible.
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