Sample collection and isolation Automated cfDNA Bead Extraction Kit
Bead Xtract cfDNA 680x680

Scalable, automatable cell-free DNA extraction

Bead Xtract cfDNA kit

A flexible and efficient cfDNA extraction kit that uses paramagnetic beads to obtain high quality cfDNA from plasma or serum.

Flexible and scalable cfDNA purification

Extraction is easily scalable to suit your laboratory needs, with a range of input volumes from 0.5-10 ml and manual and automated protocols.

Fully automated or manual protocol

From lysis through to separation, the protocol can be fully automated and is compatible with most open-source liquid handlers.

Maximize cfDNA yield for every sample

High binding capacity beads maximize cfDNA recovery and an elution volume as low as 30 µl ensures cfDNA is concentrated enough for downstream applications without the need for DNA vacuum concentration.

Quality cfDNA output for applications such as NGS

The reversible nucleic acid-binding properties of paramagnetic particles along with an optimized binding system that targets recovery of small cfDNA fragments and minimizes gDNA contamination.

Simple, scalable cfDNA extraction from plasma or serum

The utility of cell-free DNA (cfDNA) in both translational research and diagnostic settings has increased dramatically since its discovery.

Extracting cfDNA from biological samples can be challenging due to its low concentration and small fragment size. It is also critical to minimize genomic DNA (gDNA) contamination from white blood cells, to achieve accurate cfDNA analysis. Therefore a cfDNA isolation method that is highly efficient at recovering smaller fragment sizes is highly desirable.

To address this need, Nonacus have developed a flexible and scalable bead-based cfDNA extraction kit that is able to quickly and efficiently isolate high quality cfDNA from up to 10 ml plasma or serum samples.

Flexible cell-free DNA purification

The Bead Xtract cfDNA kit is simple and easy to use and is completely scalable to fit with your sample requirements. A flexible input volume of 0.5-10 ml plasma or serum avoids the requirement for multiple cfDNA extractions, saving you precious time and resources.

The Bead Xtract cfDNA protocol offers ultimate user flexibility, as it can be run manually or as a fully automated process and is highly compatible with a range of open-source liquid handlers.

automation-2 680x616

Maximize cfDNA yield

The high binding capacity of the paramagnetic particles used in the Nonacus Bead Xtract cfDNA kit maximizes cfDNA yield from plasma samples. It also reduces the volume of particles required to isolate the cfDNA so that an elution volume as low as 30 µl can be achieved even with very large volumes of plasma.

High performance cfDNA extraction


Figure 1: Average DNA yield (ng) extracted from 1 ml plasma replicates using a range of different bead-based and spin-column based extraction kits. cfDNA was extracted following the manufacturer's instructions and eluted into 50 µl of elution buffer. DNA concentration was determined using high sensitivity reagents on the Qubit 3.0 (Invitrogen).

Automated cfDNA extraction using the Bead Xtract cfDNA kit shows expected nucleosomal patterning

Figure 2: Automated cfDNA extraction using the Bead Xtract cfDNA kit shows expected nucleosomal patterning typical of cfDNA fragment distribution and low gDNA contamination. 4 ml replicates performed on the Hamilton ML STAR and eluted in 50 µl of buffer. Purified DNA was analyzed on the Agilent 4200 Tapestation

Streamlined workflow

The properties of the paramagnetic particles in the Bead Xtract cfDNA kit ensure fast magnetic separation even when using large volumes of plasma.

The elimination of funnels and vacuum steps streamlines the process so that manual extraction takes less than 70 minutes with a hands-on time of under 30 minutes.

Diagram of the automated Bead Xtract cfDNA kit workflow
Diagram of the automated Bead Xtract cfDNA kit workflow

Automated cfDNA extraction


For Research Use Only. Not for use in diagnostic procedures. 

See our customer publications

ProductCatalog No.
Bead Xtract cfDNA, 20 (1 ml) extractionsPRE_EXT_BXC_20
Bead Xtract, cfDNA 200 (1 ml) extractionsPRE_EXT_BXC_200
Bead Xtract, cfDNA 800 (1 ml) extractionsPRE_EXT_BXC_800

How should I store the plasma sample prior to cell-free DNA extraction?

How should I store my eluted cell-free DNA sample?

How can I remove any precipitates from the buffers in my extraction kit?

How can I ensure that the magnetic beads are fully resuspended in solution when using the Bead Xtract cfDNA kit?

Can I elute in water instead of the supplied buffer which contains EDTA?

Interested in our Bead Xtract cfDNA kit?