Cell3™ Target: Whole Exome Enrichment
Exome sequencing and nothing more…
Exome enrichment that delivers content focused on what matters, allowing you to reduce sequencing costs and improve throughput.
Focused exonic content
Our optimised exome covers 33Mb of highly-conserved protein coding regions and 99% of ClinVar variants, so you can focus on what matters.
Optimised performance
Delivering excellent uniformity of coverage, Cell3™ Target Whole Exome minimises low coverage regions reducing sequencing costs and improving sample throughput
Range of sample types
Cell3™ Target Whole Exome supports a broad range of sample types, including cell free, genomic and formalin-fixed, paraffin-embedded (FFPE) DNA.
Customisable
Designed to be flexible, Cell3™ Target Whole Exome allows you to add extra content to cover targets specific to your project.
Whole Exome enrichment
Whole exome sequencing has been widely adopted in the last decade as an efficient way of screening the genome for disease-associated mutations. By focusing reads on coding regions, which contain more than 80% of known disease-causing variants, the probability of identifying mutations associated with disease is increased. At the same time, the amount of sequencing (and analysis) required is reduced by 99% when compared to whole genomes, significantly reducing the cost of sequencing. This makes whole exome sequencing an efficient and cost-effective alternative to whole-genome sequencing especially in clinical applications.
Exome content focused on what matters
The Cell3™ Target Whole Exome focuses on the core protein-coding regions referenced in CCDS. Using a 33Mb design (37Mb sequencing footprint) covering 99% of ClinVar variants, we achieve coverage of >97% of targeted regions at >= 20x coverage with a 150x mean sequencing depth and requiring just 4.90Gb of sequencing per sample.
By focusing on what matters, the Cell3™ Target Whole Exome offers you a cost effective and efficient whole exome solution and the choice of running more samples or samples at greater depth, than other exome products.
Optimised sequencing performance
The baits used in Cell3™ Target Whole Exome are designed to deliver excellent uniformity of coverage. By improving uniformity of coverage and reducing the number of low coverage exons, our whole exome enrichment optimises sequencing efficiency and sample capacity per sequencing run.
Broad range of sample types including FFPE
We know that you may want to use the same exome product on multiple sample types – especially for testing matched samples. The Cell3™ Target Whole Exome kit has been developed for and validated on broad range of sample types, including cell free (circulating tumor (ctDNA) and cell-free fetal (cff DNA)), genomic, and both fresh frozen and formalin-fixed, paraffin-embedded (FFPE) DNA.
Using from as little as 1 ng of DNA with any of these sample types, Cell3™ Target Whole Exome unlocks the door for prenatal and oncology applications
Figure 1. Using UMI’s to identify and quantify individual DNA molecules during library preparation increases sensitivity
Detect low frequency mutations for prenatal or cancer research
The Cell3™ Target library preparation behind our whole exome enrichment incorporates error suppression technology. This includes unique molecular indexes (UMIs) and unique dual indexes (UDIs), to remove both PCR and sequencing errors and index hopping events. This error suppression technique, combined with our excellent uniformity of coverage, allows you to confidently and accurately call mutations down to 0.1% VAF (and that’s from as little as 1 ng ctDNA input).
Customisable content on whole exome products
Designed to be flexible, our Cell3™ Target Whole Exome allows you to add extra content specific to your project. Whether this is additional content or increased coverage of existing content, our Probe Design Tool makes this a simple and easy process to implement. And our rapid production turnaround means you will receive a fully NGS-validated custom exome within 4 weeks.
- Additional content with high enrichment uniformity
- Increased coverage of specific genes covered by Cell3™ Target Whole Exome
- Optimization of spike-in ratio
Log into the Probe Design Tool at My Nonacus to start the design process and get a quote or drop us an email at info@nonacus.com
Product Resources
Detailed product information available to download.
For Research Use Only. Not for use in diagnostic procedures.
Product Support
If you have a query relating to any of our products, please take a look at our FAQs or complete our Contact me form and one of our team will get back to you as soon as possible.
Learn more about our other exome products
ExomeCG
Clinically enhanced exome capture for whole exome sequencing and copy number analysis in a single test
Tumor Exome
Exome capture designed for cancer research covering key intronic regions like promoters and genome-wide coverage of CNVs